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KMID : 1094720130180061101
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Biotechnology and Bioprocess Engineering
2013 Volume.18 No. 6 p.1101 ~ p.1108
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Streamlined cell-free protein synthesis from sequence information
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Ahn Jin-Ho
Lee Kyung-Ho
Shim Jae-Won
Lee Eun-Yeol
Kim Dong-Myung
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Abstract
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In this study, we describe a cell-free protein synthesis consolidated with polymerase chain reaction (PCR)-based synthetic gene assembly that allows for streamlined translation of genetic information. In silico-designed fragments of target genes were PCR-assembled and directly expressed in a cell-free synthesis system to generate functional proteins. This method bypasses the procedures required in conventional cell-based gene expression methods, integrates gene synthesis and cell-free protein synthesis, shortens the time to protein production, and allows for facile regulation of gene expression by manipulating the oligomer sequences used for gene synthesis. The strategy proposed herein expands the flexibility and throughput of the protein synthesis process, a fundamental component in the construction of synthetic biological systems.
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KEYWORD
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cell-free protein synthesis, synthetic biology, high-throughput expression, synthetic gene, translation
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